Composite
Part:BBa_K4354008
Designed by: Java Yao Group: iGEM22_GXU-China (2022-07-28)
BioBrick used to express enzymes about total nitrogen removal and report gene GFP
Plasmid pHY-P43 is a expression vector from Bacillus subtilis. We choose it to express specific genes in our engineering Bacillus subtilis. It has ampicillin resistance and tetracycline resistance. It is a medium-low copy vector about 5166bp.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 501
Illegal EcoRI site found at 986
Illegal EcoRI site found at 4660
Illegal PstI site found at 1851
Illegal PstI site found at 3007
Illegal PstI site found at 3013
Illegal PstI site found at 3771
Illegal PstI site found at 5066 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 501
Illegal EcoRI site found at 986
Illegal EcoRI site found at 4660
Illegal NheI site found at 2987
Illegal PstI site found at 1851
Illegal PstI site found at 3007
Illegal PstI site found at 3013
Illegal PstI site found at 3771
Illegal PstI site found at 5066 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 501
Illegal EcoRI site found at 986
Illegal EcoRI site found at 4660
Illegal BglII site found at 2572
Illegal BamHI site found at 1687 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 501
Illegal EcoRI site found at 986
Illegal EcoRI site found at 4660
Illegal PstI site found at 1851
Illegal PstI site found at 3007
Illegal PstI site found at 3013
Illegal PstI site found at 3771
Illegal PstI site found at 5066 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 501
Illegal EcoRI site found at 986
Illegal EcoRI site found at 4660
Illegal PstI site found at 1851
Illegal PstI site found at 3007
Illegal PstI site found at 3013
Illegal PstI site found at 3771
Illegal PstI site found at 5066
Illegal NgoMIV site found at 4235
Illegal NgoMIV site found at 4427
Illegal AgeI site found at 4814
Illegal AgeI site found at 5431
Illegal AgeI site found at 5873 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 884
Illegal BsaI.rc site found at 1696
Illegal BsaI.rc site found at 5183
Illegal BsaI.rc site found at 5462
Illegal BsaI.rc site found at 6567
Illegal SapI.rc site found at 1839
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Categories
Parameters
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